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Experiment 1 - Coggle Diagram
Experiment 1
Experiment 2
Experiment 3
Experiment 4
Experiment 5
Mammalian Two Hybrid
Construction of Vectors
Transfection
Selection
Obtain results & analyze
Western Blot
Set up SDS page & perform electrophoresis
Transfer membrane & block with blocking buffer
Stain with antibody
Detect using method of choice: Chemiluminescent
Coimmunoprecipitation
add antibody that bind with antigen into protein mixture
add solution into tube & ultracentrifuge
remove supernatant
analyze pellet via SDS-PAGE
1 band = interaction
2 or more bands = no interaction
CDsearch
Enter protein sequence
Analyze result & identify domains
BlastP
Enter protein sequence in FASTA format
Choose 'pdb' in database & Enter organism of choice
Click Blast & wait for results
Analyse results