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BLAST-P, The primary amino acid sequence of Protein Awesome1: …

- - - - CO-IP Western Blot
- - - - ExPASy
        
        To determine pI and molecular weight (MW) of Protein Awesome1.
        
        2D gel and tandem MS
        
        To determine possible post translational modifications (PTMs) in Protein Awesome1.
      - To identify protein-protein interactions (PPI)
        
        PPIs can tell us about the possible role of protein Awesome1 in the biological processes in the cell.
    - - Most PTMs occur in the endoplasmic reticulum (ER) and Golgi apparatus. Thus if protein Awesome1 is present in the ER/Golgi, we can predict that it would have PTMs.