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Molecular Fluorescence - Coggle Diagram
Molecular Fluorescence
Theories
Electronic excitation of molecules by absorption of radiation
Jablonski diagram
Vibrational relaxation
Internal conversion
Intersystem crossings
Strokes shift
Rate of relaxation events
Relationship of fluorescence intensity to the Beer-Lambert law
Instrumentation for molecular fluorescence
Xenon lamp
Arc lamp
Lasers
Pumping
Spontaneous emission
Stimulated emission
Resonance
Amplification- Positive Gain
The laser beam
Type of lasers
Adjustable slits
Excitation monochromator
Photon detector
Process
Photodiode
Avalanche Photodiode
Excitation correction
Sample compartment
Emission monochromator
Fluorescence Quenching
Four general types of quenching process
Dynamic (Collisional)
Static (Pre-Association)
Static (Perrin)
Energy transfer to a Non-Emissive species
Dexter energy transfer
Forster Energy transfer