Photosynthesis

Chloroplast

Two stages of photosynthesis

C4 plants

Energy within light is captured and used to synthesize carbohydrates

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CO2 + H2O + light energy → C6H12O6 + O2 + H2O

CO2 is reduced

H2O is oxidized

Energy within light is captured and used to synthesize carbohydrates

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Organelle in plants and algae that carries out photosynthesis

Green pigment is chlorophyll

Majority of photosynthesis occurs internally in leaves, in the mesophyll

Carbon dioxide enters and oxygen exits leaf through pores called stomata

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Chloroplast anatomy

Outer and inner membrane separated by intermembrane space

A third membrane, the thylakoid membrane contains

Membrane forms thylakoids

Enclose thylakoid lumen

Granum – stack of thylakoids

Fluid filled region between thylakoid membrane and inner membrane is the stroma

pigment molecules

Light reactions

Calvin cycle

Use light energy

Take place in thylakoid membranes

Produce ATP, NADPH and O2

Occurs in stroma


Uses ATP and NADPH to incorporate CO2 into carbohydrate

Photosynthetic pigments

Leaves are green because they absorb red and violet, and reflect green wavelengths

Wavelength of light that a pigment absorbs depends on the amount of energy needed to boost an electron to a higher orbital

Pigments absorb some light energy and reflect others - Having different pigments allows plants to absorb light at many different wavelengths

Evolved a mechanism to minimize respiration

C4 plants make oxaloacetate (4 carbon molecule) in the first step of carbon fixation

Hatch-Slack pathway

Leaves have two-cell layer organization

Mesophyll cells

CO2 enters via stomata and 4 carbon compound formed (PEP carboxylase does not promote photorespiration)

Bundle-sheath cells

4 carbon molecule transferred that releases steady supply of CO2, minimizing photorespiration

CAM plants

Some C4 plants separate processes using time

Crassulacean Acid Metabolism

CAM plants open their stomata at night

CO2 enters and is converted to malate

Stomata close during the day to conserve water

Oxaloacetate converted to malate

Malate broken down into CO2 to drive Calvin cycle during the day

Molecular Features of Photosystems

Photosystem II (PSII)

Two main components:

Light-harvesting complex (or antenna complex)

Directly absorbs photons

Energy transferred via resonance energy transfer

Reaction center

P680 →P680*

P680* is relatively unstable, so energy is transferred quickly

Electron transfers to primary electron acceptor and captured

Water is oxidized to replace the electron on P680+, producing oxygen gas in the process