Photosynthesis
Chloroplast
Two stages of photosynthesis
C4 plants
Energy within light is captured and used to synthesize carbohydrates
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CO2 + H2O + light energy → C6H12O6 + O2 + H2O
CO2 is reduced
H2O is oxidized
Energy within light is captured and used to synthesize carbohydrates
Organelle in plants and algae that carries out photosynthesis
Green pigment is chlorophyll
Majority of photosynthesis occurs internally in leaves, in the mesophyll
Carbon dioxide enters and oxygen exits leaf through pores called stomata
Chloroplast anatomy
Outer and inner membrane separated by intermembrane space
A third membrane, the thylakoid membrane contains
Membrane forms thylakoids
Enclose thylakoid lumen
Granum – stack of thylakoids
Fluid filled region between thylakoid membrane and inner membrane is the stroma
pigment molecules
Light reactions
Calvin cycle
Use light energy
Take place in thylakoid membranes
Produce ATP, NADPH and O2
Occurs in stroma
Uses ATP and NADPH to incorporate CO2 into carbohydrate
Photosynthetic pigments
Leaves are green because they absorb red and violet, and reflect green wavelengths
Wavelength of light that a pigment absorbs depends on the amount of energy needed to boost an electron to a higher orbital
Pigments absorb some light energy and reflect others - Having different pigments allows plants to absorb light at many different wavelengths
Evolved a mechanism to minimize respiration
C4 plants make oxaloacetate (4 carbon molecule) in the first step of carbon fixation
Hatch-Slack pathway
Leaves have two-cell layer organization
Mesophyll cells
CO2 enters via stomata and 4 carbon compound formed (PEP carboxylase does not promote photorespiration)
Bundle-sheath cells
4 carbon molecule transferred that releases steady supply of CO2, minimizing photorespiration
CAM plants
Some C4 plants separate processes using time
Crassulacean Acid Metabolism
CAM plants open their stomata at night
CO2 enters and is converted to malate
Stomata close during the day to conserve water
Oxaloacetate converted to malate
Malate broken down into CO2 to drive Calvin cycle during the day
Molecular Features of Photosystems
Photosystem II (PSII)
Two main components:
Light-harvesting complex (or antenna complex)
Directly absorbs photons
Energy transferred via resonance energy transfer
Reaction center
P680 →P680*
P680* is relatively unstable, so energy is transferred quickly
Electron transfers to primary electron acceptor and captured
Water is oxidized to replace the electron on P680+, producing oxygen gas in the process