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Pack 16 - Photosynthesis, Respiration and ATP, Glucose, ATP, ATP, Pyruvate…
Pack 16 - Photosynthesis, Respiration and ATP
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Respiration
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Anaerobic Respiration
Glycolysis
The splitting of one molecule of glucose (6C) into two molecules of pyruvate (3C) - Occurring in the cytoplasm of the cell
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Fermentation
In anaerobic respiration, the pyruvate produced in glycolysis is concerted into ethanol (in plants and yeast) or lactate (in animals and some bacteria) using reduced NAD - reducing NAD allows for glycolysis to continue and a small amount of ATP to still be produced
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Link Reaction
Pyruvate is Decarboxylated (one carbon atom is removed in the form of CO2) and is oxidised to form acetate and NAD is reduced to form reduced NAD
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Krebs Cycle
Series of oxidation-reduction reactions occurring in the mitochondrial matrix, the cycle happens once for each pyruvate molecule so twice for each glucose
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Definitions
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When light energy "excites" electrons in an atom or molecule, increasing energy causing them to be released. release of electrons causes atom or molecule to become a positively-charged ion
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A series of small reactions controlled by enzymes, eg. respiration and photosynthesis
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Adding phosphate to a molecule, eg ADP > ATP
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Coenzymes
A molecule that aids the function of an enzyme. Work by transferring a chemical group from one molecule to another.
In Photosynthesis - NADP, transfers hydrogen, reduces itself
In Respiration - NAD, coenzyme A and FAD, NAD + FAD transfer hydrogens, coenzyme A transfers acetate
ATP
Cells can't get energy directly from glucose so energy released from glucose in respiration is used to make ATP (adenosine triphosphate). ATP carries energy to where its needed
ATP is synthesised via a condensation reaction between ADP (adenosine diphosphate) and inorganic phosphate (Pi)- using energy from releasing reactions, the energy is stored as chemical energy in the phosphate bond. The enzyme ATP Synthase catalyses the reaction
ATP diffuses into parts of the cell that need energy. It is hydrolysed back into ADP + Pi releasing the chemical energy. The enzyme ATP Hydrolase catalyses the reaction.
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Glucose is phosphorylated using a phosphate from ATP producing 1 molecule of glucose phosphate and ADP
ATP is used again to add another phosphate, forming hexose bisphosphate
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This energy is used to transport protons into the thylakoid = the thylakoid has a higher concentration of protons than the stroma. This forms a proton gradient across the thylakoid membrane.
Protons move down the concentration gradient, into the stroma, via the enzyme ATP synthase, which is embedded in the thylakoid membrane. The energy from this movement combined ADP with inorganic phosphate to form ATP
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It combines with RuBP a 5 carbon compound, reaction is catalysed by the enzyme rubisco
This produces an unstable 6-carbon compound which quickly breaks down into 2 molecules of a 3-carbon compound called GP
The hydrolysis of ATP provides energy to turn the 3-C compound into a different 3-carbon compound called triose phosphate
The reaction also requires H+ ions, which comes from the reduced NADP
Some triose phosphate is then converted into useful organic compounds (glucose) and some continue in the cycle to regenerate RuBP
Five out of six molecules of triose phosphate produced in the cycle aren't used in making hexose sugars, but to regenerate RuBP
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Triose phosphate is oxidised forming 2 molecules of pyruvate, NAD collects the hydrogen ions reducing it. 4 ATP produced bit 2 were used in stage one so net gain of 2 ATP
In aerobic respiration The 2 molecules of reduced NAD go to oxidative phosphorylation, the 2 pyruvate are actively transported into the matrix of the mitochondria
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