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DNA recombination - Coggle Diagram

- - - - made of 400 amino acids, 7 sequence motifs
      - have a zinc binding domain and a winged helix involved in replication fork recognition
      - Helicase-and-RNase D-C terminal contributes to DN Abinding
    - - catalyses Holliday junction branch migration and fork regression
      - it disrupts the structure that can impede replication since it can unwind 3'-5' , since it forms a complex with topoisomerase III
  - - - holds the Holliday junction in the X orientation
      - a tetramer of RuvA binds to Holiday junction DNA with high specificity
      - assembles 2 RuvB rings on opposing strands which unwinds the Holiday junction
    - - performs branch migration, to the preferred location for RuvC
      - stabilises junction
    - - an endonuclease that resolves the Holliday junction, by inducing paired cleavages across the junction
      - attaches when RuvA detaches
      - folds Holiday junction and cleaves across point of strand exchange
    - - hexamer ring of RuvB threads onto dsDNA
      - functions as a specialised helicase
    - - stabilises complex
- - - - formation of gaps
      - Ss or dsDNA break
      - exposure of ssDNA region by removing nucleotides using enzymes
    - - homologous strands paired up and strand exchange as 3' end invades and displaces the unbroken duplex of another DNA molecule
      - produces 4-stranded branches DNA intermediates
      - Branch migration as invasive 3' end elongates to displace more of the unbroken strand, the displaced strand pairs up with broken DNA and is used as a template for elongation
    - - 4 stranded Holliday junction cut to give either a crossover or noncrossover
      - horizontal cleavage produces noncrossover and vertical produces crossover