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Studying cells - Coggle Diagram
Studying cells
Homogenation
Breaks open the membrane
Release the organelles
The cell is homgenated
Homogenate solution
Filtered to remove unwanted tissue
Filtrate
Ultracentrifugation
Homogante poured off
Spun at higher speeds
Most dense organelle forms pellet
Higher the speed the more dense an organelle can be
Spin filtrate at high speeds
Order
Nucleus
Mitochondria
Chloroplasts
Lysosomes
Ribosomes
Cell fractionation
Isotonic
Same water potential
Prevents osmosis
Stops the cell bursting or rupturing
Buffer
Same pH
Prevents the cell being denatured
Prevents structure changing
Cold
Prevents enzyme action
Stops cell changing after breaking apart
Microscopy
Magnification
Magnification = size of image/size of real object
Resolution
Minimum distance for two object to appear as separate