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Methods of preparation of Transgenic Animals, Purification of transgene…
Methods of preparation of Transgenic Animals
Harvesting zygotes or fertilized eggs from female mice
Construction of a transgene
Purification of transgene DNA
Method
Gene knockout and RNA
Embryonic stem cell transfer
Retrovirus-mediated gene transfer
Microinjection
CRISPR or CAS9
Implantation of microinjected zygotes into recipient mice
Pregnant mice delivered pups
PCR screening of transgenic positively by pups
20 days postimplantation
10 days post delivery
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