Please enable JavaScript.
Coggle requires JavaScript to display documents.
TRANSGENIC ANIMALS - Coggle Diagram
TRANSGENIC ANIMALS
METHOD
CONSTRUCTION of a Transgene
purification of a transgene
harvesting a zygote or fertilized eggs from females
introduction of a transgene in zygote
zygote with transgene is implanted into mother
transgeneic animal is produced
Methods
microinjection of transgene
This method involves the direct microinjection of a chosen gene construct into the pronucleus of a fertilized ovum.
Efficiency is low
1 to 5 transgenic animal out of 100 born
dolly the sheep was first that is produced by this method
Nuclear transfer technique
It involves removing the DNA from an oocyte (unfertilised egg), and injecting the nucleus which contains the DNA to be cloned.
efficiency is high approximately 100%
Retrovirus mediated gene transfer
transgenic animals are produced by inserting a foreign gene into its genome.
first transgenic animal mice
applications
Increased milk production
Increased livestock Production
Wool and leather are the major byproducts that could be altered by genetic engineering
To influence the immune response and increase the disease resistance capacity of livestock
To increase the reproductive performance of farm animals
Drugs and industrialproduction
Medicinal approach
Xenotransplantation
Blood replacement