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Methods for preparation of transgenic animals, viral vector use - Coggle…
Methods for preparation of transgenic animals
DNA microinjection.
direct physical approach
injection into the pronucleus of a fertilized ovum.
delivery of DNA into cell -microinjection
for variety of species
first technique
<10% newborn with transgene
Embryonic stem cell-mediated gene transfer.
Make your DNA
Transform ES cells in culture
Inject into (IMC) blastocysts.
5.Embryo transfer
6.test offspring
Select for successfully transformed cells
Retrovirus-mediated gene transfer.
virus or a plasmid use
Retroviruses
Offspring - chimeric
Chemical Transfection
calcium phosphate
DNA cross-membrane transport
Nuclear Transfer Technique
DNA transfer to culture media
nucleus transfered to enucleated egg
90 -100% newborn with transgene
viral vector use