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histo1: membranous cell organelle - Coggle Diagram
histo1: membranous cell organelle
cell= cytoplasm + nucleic+ cell membrane
cell membrane
EM: trilamllar 2 electron dense + 1 lucent
can't seen by LM H&E - PAS
protein 60%+lipid 35%+CHO 5%
lipids
cholesterol ---> found in the center (cytoplasmic side)
phospholipid = 2 hydrophobic tails to the center + 1 polar hydrophilic head outside = bilayer----> channels for lipid soluble substances
proteins
peripheral = extrinsic ---> loosely attached
integral = intrinsic
small particles partially embedded
trans membranous wholly embedded
CHO
glycoprotein & glycolipids
cell coat = fuzzy moderate electron dense granule + PAS magenta color
RIA= recognition, receptor, immunity, adhesion
functions
Bulk transport: exocytosis + endocytosis
RIA
cytoplasm = matrix+ cell organelles (membranous- non membranous) + cell inclusion
mitochondria
power house of energy ATP
present more in active cells:liver > less in lymphocytes
LM: H&E= no stain \ iron H = dark blue \ jenous green= green \ histochemically = succinic dehydrogenase
EM: outer smooth layer permeable+ inner layer shelf like (cristae) contains respiratory & electron transport enzymes
negative stain image---> elementary particles which contains phosphorylation enzymes
matrix: DNA & RNA of mito + electron dense granules Ca&Mg + Kreb's cycle enzymes
Function: power house + division of mito + contains enzymes + B oxidation of short fa
ER= anastomosing tubules & cisternae
sER
lipid forming protein ---> liver
not seen
anastomosing tubules - smooth due to the absence of ribosomes
detoxification of drugs in the liver+ glycogen breakdown+help in muscle contraction + synthesis of lipids
rER
protein forming cells:plasma cells
basophilic due to ribosomes
rough due to ribosomes + flattened membranous cisternae
F: glycosilation of proteins + packing proteins +segregation of protein + transfer protein with vesicles to golgi
lysosomes = acid hydrolase intracytoplasmic digestion
not seen by H&E , histochemically = phosphatase activity
present in all cytoplasm, more abundant in phagocytic cell
EM: primary = homogenous moderate electron dense + secondary = heterogenous electron dense
autophagic vacuoles ----> unuseful bodies (lipo fuscia pigment)
multivesicular ---> pinocytotic vesicular
heterolysosomes --> phagosomes ( exocytosis)
functions: digestion + defensive mechanism + removal of old organelles + inactive ---> active hormones + penetrate ovum
golgi= secretory system
negative golgi image ----> silver stain
4-10 saccules (flattened membranous cisternae) ----> stack
concave face = trans mature + convex = cis immature
micro vesicles = transfer (small - coming from rER to the convex face) + macro vesicles = secretory (large - exocytosis or to the lysosomes)
functions: modifies protein by adding CHO+ modification of the execratory particles+ makes the lysosomes + recycle the CM + sorting out proteins
peroxisomes
contains: D amino acid oxidase + hydroxy oxidase + uricase enzyme + catalase enzyme
not seen by H&E ---> histochemically = catalase enzyme
in human: homogenous electron dense granules+ in rodents: e d semicrystalline
functions: segregate of H2O2+ B oxidation product of long chain fa + formation of bile acid + impo role in metabolism of alcohol