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transgenic animals - Coggle Diagram
transgenic animals
physical transfection
microinjection: mesenchymal stem cells or smooth muscle cells
optical transfection
biolistic transfection
electroporation
chemical transfection:
Ca2+phosphate
polycations
dendrimers
calcium chloride
Bactofection
spontaneous bacterial lysis in the infected cells cytosol
cell-to-cell spread bacterias.
To express plasmid-encoded heterologous proteins
retrovirus mediated gene transfer:
cDNA is recovered by genomic PCR.
293-based packaging cell lines
Construction of a cDNA library
artificial chromosome transfer:
fusion of microcell with recepient cell.
integrating transferred chromosome into host cell
induction of micronuclei in donor cells.