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METHODS OF CREATION OF TRANSGENIC ANIMALS - Coggle Diagram
METHODS OF CREATION OF TRANSGENIC ANIMALS
VECTOR MEDIATED
RETROVIRAL VECTOR
RETOVIRUSES USED AS A VECTOR CHIMERAS ARE PRODUCED
COSMID VECTOR
BACTERIOPHAGE VECTOR
PLASMID VECTOR
NON VECTOR MEDIATED
DIRECT METHOD
MICROINJECTION
1.THIS METHOD INVOLVES THE DIRECT MICROINJECTION OF A CHOSEN GENE CONSTRUCT
2.ITS INOCULATION INTO PRONUCLEUS OF FERTILIZED OVUM.
3.THE FERTILIZED OVUM IS TRANSFERRED INTO THE OVIUCT OF FEMALE RECEPIENT.**
EMBRYONIC STEM CELL MEDIATED GENE TRANSFER
1.EMBRYONIC STEM CELLS
2.FORMATION OF GAMETOCYTES 3.INJECTION INTO BLASTOCYSTS 4.INJECTION INTO FOSTER MOTHER 5.FORMATION OF NEW INDIVIDUAL
ELECTROPORATION
SOMATIC CELL NUCLEUS TRANSFER/ PRONUCLEUS METHOD
1.FORMATION OF DNA
2.FUSION OF MALE PRONUCLEUS WITH DESIRED DNA.
3.FORMATION OF DIPLOID ZYGOTE. 4.MITOTIC DIVISION. 5,FORMATION OF NEW INDIVIDUAL.
LASER METHOD
INDIRECT METHOD
CALCIUM PHOSPHATE DNA CO-PRECIPTATION
The calcium phosphate transfection method for introducing DNA into mammalian cells is based on forming a calcium phosphate-DNA precipitate.
Calcium phosphate facilitates the binding of the DNA to the cell surface. DNA then enters the cell by endocytosis.
DEAE-DEXTRAN MEDIATED
Diethylaminoethyl (DEAE)-dextran is a polycationic derivative of the carbohydrate polymer dextran, and it is one of the first chemical reagents used to transfer nucleic acids into cultured mammalian cells