Please enable JavaScript.

Coggle requires JavaScript to display documents.

TRANSGENIC ANIMALS Genetically modified organisms in which there has been…

- - - - creating transgenic organism
      - introduction of target gene directly into single cell
      - no req. of marker gene
  - - - pipette sucks target cell at tip.
        
        injected through membrane of cell
        
        deliverance of content of needle into cytoplasm
  - - - Make your DNA using recombinant DNA methods
        
        Transform ES cells in culture
        
        Select for successfully transformed cells
        
        Inject these cells into the inner cell mass (ICM) of mouse blastocysts
        
        Embryo transfer
        
        Test her offspring
        
        1 more item...
  - - - target gene located and isolated using restriction enzyme, transferred to retrovirus
        
        Retrovirus RNA contains target gene
        
        retrovirus infect target organism
        
        cells allowed to culture
        
        cells containing target gene are removed and inserted into embryo nucleus
        
        insertion of embryo into mammal
        
        1 more item...
    - - ability to stably integrate into the target cell genome
      - simple procedure for recombinant virus preparation
    - - random integration into host chromosome, resulting in possible insertional mutagenesis or oncogene activation