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cell confinement in vivo - Coggle Diagram
cell confinement in vivo
physical limits on cell motility
nucleus - rate limiting role in confined migration
tumor cell arrest in collagen pores less than 10% of nuclear cross section area (7µm2)
modulation of nuclear stiffness - knockdown lamins, enhances transmigration in transwell assay
interenstingly, cells at the periphery of the tumor display softer nuclei
in pores larger than 7µm2, migration is lamin independent
affects tumor cell trafficking
endosomal sorting complexes required for transport and actin filament nucleator protect the nucleus
confined migration may cause DNA damage
matrix remodeling with small pores
cells can use MMP to cleave fibers
MMMP independent manner - MTLn3e deform collagen via Rho-ROCK
MMP independent migration may happen in collagen without crosslinks
non metastatic parental MTC cells do not deform collagen
cell motility through preexisting tracks
no need for MMPs
Gm6001 MMP inhibitor
confined migration mechanisms
pseudopodial migration
matrix adhesion required
In 3D, fan like protrusions at the distal ends of pseudopodia
polarization of cortactin, WASP, RAC1, CDC42, PIP3
cell contractility also facilittates pseudopodial migration
lobopodial migration of fibroblasts
bleb based amoeboidal migration
protrusion based amoeboidal migration under low contractility
osmotic engine model
cell navigation through complex topographies
physical confinement effects
multi to single cellular transitions
cell signalling
cell division and gene expression