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Sample number& Group, Group 1, Group 2, Group 3, Group 4, CFSE- cells…
Sample number& Group
CFSE -
DB+
DB-
CFSE+
DB+
DB-
Cell count for Friday
Sample 16
Group 1
Sample 1, 4
Group 2
Sample 5, 6
Group 3
Sample 2,3, 10-15
Group 4
Sample 7, 8, 9
CFSE- cells required:
2x 5e5 +5e5 (Extra) =
1.5e6
CFSE+ cells required
3x 1e6 + 5e5(Extra) =
3.5e6
CFSE- cells required:
2x 5e5 +5e5 (Extra) =
1.5e6
CFSE+ cells required
8x 5e5 + 5e5(Extra) =
4.5e6
CFSE- cells required:
0.5e6
Total: 8e6
Total: 3e6
Overall: 1.15e7 cells required