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GOAL:To identify natural fungicides from Syllibium marianum against…
GOAL:To identify natural fungicides from Syllibium marianum against Macrophomina phaseolina (Charcoal rot pathogen)
- In Vivo assessment of aqueous extracts or dry biomass of S. marianum for charcoal rot management.
plant biomass would be incorporated in soil infected with plant pathogen in different concentrations like 1%,1.5%,2%....control would be without fungal amendment
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best concentration of plant biomass could be used by farmers directly and it is a cost effective and environment friendly approach
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- Structure elucidation of potential antifungal agents from extracts of different parts of S. marianum with help of GC-MS analysis
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fractions (n-Hexane, chloroform, methyl acetate and n-butanol)showing highest antifungal activity would be selected for GC-MS analysis
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- Screening of different parts of Syllibium marianum against M. phaseolina
collection of Syllibium marianum from barren land seperate stem, root, flowers and leaves and air dried *extracts of each part are prepared in methanol
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plant part showing highest reduction in fungal biomass of macrophomina would be selected for fractionationation with polar to non polar solvent so that maximum no of antifungal compounds are extracted
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isolation and identification of Charcoal rot pathogen from infectef samples of mung bean
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