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Chlorella sorokiniana characterization - Coggle Diagram
Chlorella sorokiniana characterization
Taxonomic - algaebase.org
Classification:
Empire Eukaryota
Kingdom Plantae
Subkingdom Viridiplantae
Infrakingdom Chlorophyta infrakingdom
Phylum Chlorophyta
Subphylum Chlorophytina
Class Trebouxiophyceae
Order Chlorellales
Family Chlorellaceae
Genus Chlorella
Based on their pigmentation, morphology and membrane distribution of phosopholipids, microalgae are classified as Chlorophyta (green algae), Rhodophyta, Haptophyta, Straminophiles or Dinophyta [14].
Photoautotrophic organisms
Utilize sunlight to metabolize carbon dioxide (CO2) and water with the release of oxygen (O2): CO2 + H2O + Sunlight --> [CH2O]n + O2
Organic compounds like glucose, starch ([CH2O]n) form the building blocks for algal growth in the process of photosynthesis [27] [28].
Growth curve
Algae follows a similar growth pattern to bacteria. The growth pattern has been defined based on growth phases as shown in figure 2.4 i.e. the lag phase: Initial period of slow growth where the cells get adapted to the new environment; linear phase: period of rapid growth and faster cell division; linear phase: period of growth where light is limited; stationary phase: Period during which the growth requirement for cell division is limiting and amount of cells produced is equal
Effect of temperature
The optimum temperature for the growth of Chlorella vulgaris ranges between 25°C to 30°C where 30°C is the optimum growth temperature [37]. Cultivation of algae at 35°C has been reported to elevate lipid content to 22.7 % and increased the accumulation of neutral lipids in A. dimorphus to 59% of the total lipid content [30].
Nutrient requirement
Optimization of culture medium has been carried out to study the nutrition requirement of algae to increase the biomass quality and yield. Nutrients are important in altering the biochemical constituents of the cell. Factors such as nitrogen, phosphorous and salinity have been reported to be the major nutrient stress inducers. Nitrogen has been considered as the most critical nutrient which affects the lipid metabolism [38] [16]. Carbon, nitrogen, and phosphorous are supplied in accordance to the Redfield ratio of [106:16:1] (C: N: P) [39].
Respiration
Algae are photosynthetic microorganisms like plants and follow a circadian cycle which involves light cycle/ photosynthesis cycle and night cycle /respiration cycle. During the day, photosynthesis is predominant over respiration where energy is used to produce biomass. At night, algae use up the carbohydrates that are synthesized during the day for maintenance and production of proteins and cellular components increases [42].