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Molecular characterization and Plant Transformation of citrus - Coggle…
Molecular characterization and Plant Transformation of citrus
morphological characterization of pathogen
how the cultural bacteria will be
characterized bybiochemical markesr
how the pathogens will be identified by morphological parameters
how the pathogen will be charcterized
Synthesis of gene
how the gene will be synthesized
primer design for its confirmation
how the expression cassette will be synthesized
Molecular characterization of pathogen
PCR will be used
how the primers will be synthesized.
incorporation of expression cassette in agrobacterium
how expression cassette will be incorporated in plant vector
confirmation of transformation by PCR
plant transformation
seed sterilization
incorporation of plant expression casette
confirmation of Plant transformation
by PCR
by qPCR