CHEMICAL METHODS OF CELL DISRUPTION, image, image, image, image, image,…
CHEMICAL METHODS OF CELL DISRUPTION
In osmotic lysis (or shock), cells are suspended in a hypotonic extracellular environment, usually a dilute sucrose solution, causing them to take on water, swell and burst
The method of cell disruption selected depends on the product, scale and cell type and must obtain the necessary components effectively and reliably without disturbing any of its components.
Surfactants (surface acting agents), often called detergents, are compounds that lower surface tension and disrupt the distinct interface between hydrophobic and hydrophilic systems
Detergents used for disrupting cells are divided three categories depending on their electrical charge: anionic, cationic and non-ionic
Chaotropic agents such as urea, guanidine and sodium iodide are capable of bringing some hydrophobic compounds into aqueous solutions by disrupting the structure of water, making it a less hydrophilic environment, and weakening the hydrophobic interactions among solute molecules
In high-pressure homogenization, a cell disruption method most often used for soft, solid tissues, samples are forced through a narrow space while pressure is applied to them.
One additional method for chemical cell disruption is the utilization of chemical solvents.
Though high-pressure homogenization is the most common procedure for cell disruption, chemical methods including osmotic lysis, surfactants and chaotropic agents also are prevalent.