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Method - Coggle Diagram
Method
qRT-PCR
mRNA expression level
IL-6R
mRNA
miR-22 mimic
si-IL6R-1 (IL6R inhibitor)
Luciferase reporter assay
Binding site
of miR-22 in
IL6R
3'UTR-WT
3'UTR MUT
Flow cytometry
glucose treated/ non-treated INS-1E cell
APOPTOSIS
(%)
miR-22 mimic
si-IL6-1
Immunohistochemistry (IHC)
Observe IL-6
EXPRESSION
in pancreatic β -cells
Model
Control
Western Blot
Protein expression leve
l
JAK-STAT protein
P-JAK
P-STAT
Apoptotic protein
Bcl-2
Bax
Capase-3
CCK-8 assay
glucose treated/ non-treated INS-1E cell
VIABILITY
miR-22 mimic
si-IL6R-1 (IL-6R inhibitor)
H&E staining
Observe the
NUMBER
of pancreatic β -cells in rat pancreatic tissues
Model
Control
TUNEL staining
Observe the
APOPTOSIS
of pancreatic β -cells in rat pancreatic tissues
Model
Control