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ELISA elisa, highest sensitive, purification, false positive…
ELISA
Detect the presence of antigen in a sample
Detect the presence of antibody in a sample
SANDWICH
Detect the presence of antigen in sample
COMPETATIVE
Used to determine small molecule antigen
Quantitative
Very sensitive
Use immunological techniques
This technique is used to detect specific molecules in samples
It use an enzyme to detect the binding of Antibody or Antigen
Enzyme converts colorless substrate to colored product
Then it indicates the Ag:Ab presence
Results may not be absolute
Antibody must be available**
Concentration must be clear
False positive possible
False negative possible
Direct
Disadvantage
No signal amplification,
highest background noise
Advantage
most simple,
Fastest assay
less error prone
Indirect
Advantage
greater specificity than direct
signal amplification is possible
Disadvantage
longer Assay
.lower throughou
t
Competitive
Advantage
High sensitivity to compositional differences in complex antigen mixtures
Disadvantage
needs to use the inhibitor antige
n
relatively complex protocol
Sandwich
Disadvantage
Heterophyllic antibodies may generates a false positive signals
Advantage
Do not require purification
Highly Sensitive
Blood
Saliva
Sputum
Feces
Tissue
s
Milk
