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ELISA, IMG-20200706-WA0196 - Coggle Diagram
ELISA
Advantages
Easy to use for HIV testing
Test for antibody as well as antigen
Highly sensitive
Simple to perform
Don't need Radioactive substance
Applications
Determine Serum antibody
Track spread of disease e.g.HIV
Detect hormone level
Used in food industry
Disadvantages
Very expensive
Recognize particular particular antigens.Not all antigens
Enzyme activity may be affected
False positive/negatives possible
Types
Indirect
Direct
Competitive
Sandwich
Priciple
Direct elisa
Indirect elisa
Competitive elisa
Sandwich elisa
