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Chromatography Rishs International School 9 A By : Kailash …
Chromatography
Rishs International School
9 A
By : Kailash
Hemashree
Irfan
Manoj
Sravan kumar
Chromatography is a laboratory technique for the separation of a mixture. The various constituents of the mixture travel at different speeds, causing them to separate. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation.It may be preparative or analytical. Analytical chromatography is done normally with smaller amounts of material and is for establishing the presence or measuring the relative proportions of analytes in a mixture.
The eluate is the mobile phase leaving the column. This is also called effluent.
The eluent is the solvent that carries the analyte.
The twelve types of chromatography are:
(1) Column Chromatography
(2) Paper Chromatography
(3) Thin Layer Chromatography
(4) Gas Chromatography
(5) High Performance Liquid Chromatography
(6) Fast Protein Liquid Chromatography
(7) Supercritical Fluid Chromatography
(8) Affinity Chromatography
(9) Reversed Phase Chromatography
(10) Two Dimensional Chromatography
(11) Pyrolysis Gas Chromatography
(12) Counter Current Chromatography.
Origin
1930s: from German Chromatographie (see chromato-, -graphy), early separations being displayed as a number of coloured bands or spots.
Chromatography has various applications. It is used for the separation of different colors of ink. It is also used to identify and separate the preservatives and additives added in the food items. It is also used in DNA fingerprinting and bioinformatics.
Daily uses of Chromatography:
6.Ebola Immunisaion
Forensic testing
Drug testing
Beverage testing
Food testing
Creating vaccinations
Rf is a calculation that compares the distance the solvent traveled up the paper strip to the distance a pigment traveled up the same strip.
Chromatography
chromatography was first developed by the Russian botanist
Mikhail Tswett
in 1903 as he produced a colorful separation of plant pigments through a column of calcium carbonate.