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Cherry Basic Concept of Light Sheet Fluorescence Microscopy, my background…
Cherry Basic Concept of Light Sheet Fluorescence Microscopy
Setup形狀
orthogonal advantage:
improving axial resolution
PSF
axial resolution determine
optical sectioning
optical sectioining 好處
wide-field 的擷取速度提升
gaussian light sheet
optimal optical sectioning effect
FOV
Rayleigh length
photon bleaching / photon toxicity
two 2d slide imaging to create 3d
my background
mesoscopic; microscopic
cellular and subcellular dynamic imaging
3D imaging
in vivo imaging
tomography
direct measure
optical sectioning
inverse problem
emission / transmission
OCT
Cherry the prospect of in vivo imaging
cellular and sub-cellular dynamics event multi-dimensional imaging high resolution
development biological
long term non invasive
quantitative image
in vivo imaging
great FOV
high image aquisition speed
better spatial resolution
less damage
trade off:
FOV and psatial resolution
aquisition speed and SNR
light sheet fluorescence microscopy less comprimise