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飽思華 & Cherry 擇面光照明顯微術, 飽思華, Cherry, light sheet & inverted…
飽思華 & Cherry
擇面光照明顯微術
飽思華
光學切片技術
三維影像
共軛交螢光顯微術
多光子激發螢光顯微術
結構光照明顯微術
擇面光照明顯微術
擇面光照明顯微術
獨特的照明方式
利用圓柱透鏡製作一道位於觀測物鏡的焦平面上的高斯薄片光，使照明的激發光從側面垂直於觀測方向進入樣本，再藉由移動樣本位置，可觀測樣本不同深度影像
點掃描 缺點
light sheet 掃描方式
可旋轉的樣本台
樣本的組織結構會對光吸收及散射
可穿透深度仍較其他顯微術好，到500micron
multiview fusion
light sheet fluorescence microscopy application and development
multidirectional SPIM
SI-SPIM
Digital scanned laser light sheet fluorecence microscopy (DSLM)
Bessel beam SPIM
Two photon SPIM
Cherry
development and application
light shaping
multi-illumination
the intensity of illumination light sheets drop along the propagation direction due to scattering and absorption in samples.
sysrematic non-uniform excitation
thick tissues
pigment
dense structure
stripe shadows
scanning light sheet
multi-detection
improve image quality
aberration and blur cause by scattering downgrade image quality
multi-view concept
ultramicroscopy
OPFOS
Basic concept of light sheet fluorescence microscopy
configuration
improving axial resolution
PSF
photo-damage
fluorescence background
image aquisition time
Gaussian light sheet
optimal optical sectioning effect
waist laser beam + detection FOV; Rayleigh range & region of interest
photo-bleaching, photo-toxicity
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light sheet & inverted microscopy
optimal optical sectioning effect
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圖
large-volume image reconstruction
isotropic resolution
結合上述優點，因此SPIM非常適合透明及半透明的生物活體樣本
有時間再寫
一兩句帶過
一兩句帶過
幾句話帶過
圖
光學切片技術
先一段文字敘述，再圖說