If the concentration of particular ions, such as potassium and chloride, inside cells is measured, it is often found that they are 10–20 times more concentrated inside than outside. In other words, a concentration gradient exists, with a lower concentration outside and a higher concentration inside the cell. The ions inside the cell originally came from the external solution, therefore diffusion cannot be responsible for this gradient because, as we have seen, ions diffuse from high concentration to low concentration. The ions must therefore accumulate against a concentration gradient. The process responsible is called active transport. It is achieved by carrier proteins, each of which is specific for a particular type of molecule or ion. However, unlike facilitated diffusion, active transport requires energy, because movement occurs up a concentration gradient. The energy is supplied by the molecule ATP which is produced during respiration inside the cell. The energy is used to make the carrier protein change its shape, transferring the molecules or ions across the membrane in the process. Active transport is important in reabsorption in the kidneys, where certain useful molecules and ions have to be reabsorbed into the blood after filtration into the kidney tubules, for example.