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Genome, Genome - Coggle Diagram
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Genome
DNA
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Copying by PCR - denaturation by heating, followed by primer attachment by cooling and extension using Taq polymerase, followed by using the products for repeating cycle...
Organisation of DNA - histones, chromatid, nucleosomes, etc.
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Investigation
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ATAC-seq
Using tagmentation with hyperactive transposases, followed by PCR amplification
To find which regions are open, how open the regions are, what are the transcription binding factors, etc.
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Methylation of DNA - controls the expression of genes, and regions of DNA that are open and closed.
Pulldown method
pull down portions of DNA by detecting methylations using specific antibodies/proteins, and sequence
Bisulphite sequencing
Use bisulphite to retain only methylated cytosine, and use this to find SNPs, methylations, etc., at single nucleotide resolution.
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90% - non coding, >30% - repetitive
An open reading frame (ORF) is a continuous sequence of DNA that begins with a start codon and ends at a stop codon.
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Use of Genomics (proteins, genes, metabolites)
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To analyse disease risks (due to SNPs, splicing errors, etc.)
Protein
20 amino acids
64 codons encoding these
Multiple codons for each AA, except for tryptophan (only 1 codon)
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