Please enable JavaScript.
Coggle requires JavaScript to display documents.
Molecular Cloning, Preliminary, Procedure - Coggle Diagram
Molecular Cloning
:
- PCR amplification of insert
-
Materials
-
Primer: F and R primer, contain cutting site
DNA polymerase: enzyme, Taq PCR Supermix (2x)
-
- Digestion of vector and insert
-
-
successful transformation: cell already contain desired plasmid b/c the plate has antibody which the cell without plasmin cannot survive
-
-
- Plasmid purification: mini-prep
-
:
Insert: insertion fragment, the DNA fragment you wanna clone
-
-
-
-