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BASIC BACTERIOLOGY A, Metachromatic Staining, Shape-of-Bacterial-Cell,…
BASIC BACTERIOLOGY A
CELL STRUCTURE
Three Basic Shapes
:red_flag: :red_flag: :red_flag:
bacillus
spiral
coccus
INTERNAL TO CELL WALL
cytoplasma
inclusions
cell membrane
ribosomes
plasmid
endospores
nuceloid
CELL WALL
consist of
peptidoglikan
composed surrounding plasma membrane and
protects cell from changes in water pressure
IDENTIFICATION of BACTERIA
BACTERIAL STAINING
Fixation
2.
Kills of the microorganisms
3.
Makes it easier to stain
1.
Attach the specimen
4.
For specimen storage
Coloring microbes
with dyes that emphasizes certain structures
Types of Bacterial Staining
Simple Stain
uses only single stain:
safranin
or
methylene blue
Gram Staining
gram positive (
violet
), gram negative (
red
)
Acid-fast Staining
acid-fast bacteria (
red
), non acid-fast bacteria (
blue
)
Endospore Staining
endospore (
green
), vegetative cell (
red
)
Metachromatic Staining
metachromatic granules (
deep purple
), vegetative cell (
brown
)
