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Lab Antibiotic Sensitivity, Disinfectant, Bacteriophage - Coggle Diagram

- - - - Targets fungi
    - - Targets viruses
    - - Targets bacteria
  - - - Minimum Inhibitory Concentration
        
        Quantify sensitivity of organism to antibiotic
      - The minimum concentration required to inhibit growth of the bacteria under "standard" conditions
      - How to calculate?
        
        series of tubes set up
        
        serial dilution
        
        Same amount of bacteria
        
        Each tube having less antibiotic
        
        Nutrient broth added to each tube w/ bac & control
        
        After incubation, turbidity observed
        
        Last tube without turbidity shows the MIC
    - - Minimum Bactericidal Concentration
      - minimum concentration the prevents visible growth by inducing cell death
  - - - Zone of inhibition
    - - Categorized as
        
        resistant
        
        intermediate
        
        sensitive
    - - Ability antimicrobial to inhibit org
      - concentration of antimicrobial
      - size of antimicrobial molec
      - how quick diffuses through medium
      - number of cells on plate
      - incubation length
      - incubation temp
      - antibiotic and medium interactions
- - - - due to the multiple layers of slow growing cells in porus materials
    - - Polysaccarides
    - - P aeruginosa
        
        In Cystic Fibrosis Patients
- - - - 2 Parts
    - - Filamentus
      - Cylindrical
    - - Many sides
  - - - Transcribed and Translated
      - in a infective state
  - - - Solidifies quickly
  - - - Phage was able to lyse organism
    - - Means bacteriophage able to infect many different types of organisms
        
        "broad" host range
        
        Narrow host range
        
        able to infect a few bac strains
  - - - Phage prod enzymes to penetrate cell wall
    - - Phage binds spec site host surf
    - - Phage make large amts of viral nucleic acid and proteins
        
        using host cells machinery
    - - Assembly of viruses
        
        assembled in form of new virons
    - - Phage release enzyme to release phages outside
        
        by producing holins and lysins
        
        create pores in inner mem and break down pep glyc
  - - - Nucleid acid from phage incorporated into genome of host cell
        
        viral nucleic acid persist in host cell for many divisions
  - - - F+ and F-
        
        Can infect both cells w/ and w/o F factor
    - - F +
        
        Can only infect cells carrying F factor
    - - Def
        
        A bacteria that has incorporated DNA from a donor via conjugation
      - A transconjugant of the M13 has no plaque due to the M13 plasmid
      - The transconjugant of the T4 will have plaques, since it can infect both cells with and without the F factor
      - Effect if not properly created