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Therio Prac (Canine (What plasma prog conc that indicates the time of...,…

- - - - 5-10nmol/L
    - - 80-30nmol/L
    - - 10-18nmol/L
- - - - Registration name, number, age, breed, coat colour, markings, lip tattoos, microchip info
        Photograph
    - - past breeding results, number and type of mares, past repro evaluations, medical conditions, vacc Hx, intended future use, number of mares anticipated
    - - Musculoskeletal
        Serum (equine arteritis virus)
    - - Palpate after collection (safe): Testes, epididymis, spermatic cord
        Penis and prepuce during collection
  - - - Type of AV= Japanese (rigid, not as much water= lighter)
      - Temp of water= 43 degrees (he's excited, warm weather)= 39 degrees when serves
      - Normal n# of mounts per mating= 2 mounts 1 hr apart
    - - Volume= 50mL
      - Motility= 50% (e.g. how many moving x % moving forwards)
        
        Number of motile sperm req for preg from AI= 500 x 10(6)
        
        Number of motile sperm req for preg from natural service= 1 x 10(9)
      - Concentration= 50 million/mL
      - Buffer in extender= for lactic acid produced by sperm glycolysis (breakdown Glu)
- - - - Branding (S=SB, 2nd= WA/state, 3/4th= year)
    - - perineal conformation
        
        apposition of vulval lips
        
        Relationship with ischial bones (no more than 1/3rd above)
        
        Slop of vulval lips= >10 degrees cranial tilt
      - per rectal palpation
      - Vaginal speculum
      - Uterine culture
      - US
        
        Uterus Body= amount of Oedema
        
        Ovaries= follicles (white line around, size), CL?
  - - - Starts to be inactive
      - Oestrus decreases/ irregular
      - Going into anoestrus
      - Uterus= flaccid, ovary inactive, no follicles (decreasing hormones)
- - - - 20 million sperm/g of testis/d
        (50 ewes= 24cm,
        100= 28-30cm,
        200g +-2T, 250g +- Adult)
- - - - Manually= massage ampulla (inbetween seminal vesicles)
      - 4-6 squirts of pre-ejaculate sperm= cloudy
      - Keep everything at 37 degrees
      - Other methods= AV, EE
    - - Need dilution of 25 mill/mL to see individual sperm and asses motility etc.
      - Diff quick stained slide
        
        NE stain (alive?)
        
        Buffered Saline (phosphate)
        
        Motility etc while life
      - Volume
      - Colour
      - Density (1-5)
      - Mass activity (1-5)
      - Motility= 50% (PM + vigor 1-5)
      - Morphology= 70% (Pass= 50-69%)
        
        Head, MP, tail, PD, LH, acro, Misc.
- - - - Advance into Rectum= locate the pelvic rim
        
        No soft tissue traversing rim
        
        sweep back, retracting arm and hand to locate retroductive tract (usually ventrally)
        
        Grasp the cervix
        (firm, gristle like structure)
        
        Follow to bifurcation, feel down each horn to ovary
        on both sides
        
        Soft Tissue traversing rim
        
        Cervix (firm, sausage like)
        
        Grasp and assess movement/ weight
        
        taut with only slightly lateral movement/ none at all
        
        follow cranially to IF/ballot
        fluid filled uterus, firm foetus,
        placentomes and fremitus
        (sweep side to side)
        
        Mobile
        
        Attempt to retract empty/ early preg uterus
        back into pelvis (scooping/ refraction)
        Assess each horn and ovary
        
        Fluid filled uterus (ddx: bladder)
        
        Feel around and over and ballot foetus
        feel for roughness/ corrugation/ small spongy outgrowth of placentomes
    - - 30d
        
        uterus symmetrical, pea (30-60mL)
      - 40d
        
        asymmetrical tips of uterus, 1 finger (75-100mL)
      - 50d
        
        on pelvic rim, 2 fingers (3.5-5.5cm)
      - 60d
        
        Bulging on rim, >4 fingers, 200-400 mL fluid (mouse, 6-8cm)
      - 70d
        
        over pelvic rim, > hand and thumb, 350-650 mL both horns (7-10 cm)
      - 80d
        
        Over rim, both horn distended (8-13cm), corrugated cardboard (0.5-1cm) coty.
      - 3mnths
        
        right over edge, cant lift. 13-17cm (rat). 5 cent piece coty.
      - 4 mnths
        
        heading downwards. Small cat. Strong pulse on preg. side. 10 cent piece coty (fat feeling)
      - 5 mnths
        
        Horns stretched right down. Too big to feel (cat). Fremitus (4.5 mnths). 2.5-4cm flatter coty
      - 6 mnths
        
        starting to come back up. Smallish dog, not as mobile. Strong fremitus on preg. side (pulse on non-p). 4-5cm coty.
      - 7 mnths
        
        part of uterine body into pelvis. uterus stretched. medium dog (feel hooves- 2cm diameter). 1.25-1.5 cm fremitus on both sides. 5-7.5cm coty
      - 8 mnths
        
        Large dog at pelvis. hoof diameter= 3-4cm. 1.25-1.7cm fremitus. 6-9cm coty
      - 9 mnths
        
        Calf into pelvis, hoof diameter= 4- 6cm. 1.5-1.9 (thumb) fremitus. 8-12cm coty.