Colitis

Neonate

Differential diagnoses

Foal heat diarrhoea

Viral especially rotavirus

Salmonella

Clostridia

Necrotising enterocolitis

Sepsis

Nutritional diarrhoea

parasitic diarrhoea

Strongyloides westeri

Gastroduodenal ulceration

Foals (up to about 10-12 months)

Differential diagnoses

Cryptosporidiosis

Parasitic diarrhoea e.g. strongyles vulgaris, cyathostomins

Proliferative enteropathy e.g. Lawsonia intracellularis

Rhodococcus equi colitis

Viral especially Rotavirus

Salmonella

Clostridia

Sepsis

Nutritional diarrhoea

Gastroduodenal ulceration

Parasitic diarrhoea e.g. Strogyloides westeri

Adults

Acute

Chronic

Clinical signs: hypovolaemia, endotoxaemia

1 week duration to >1 month duration

Salmonellosis

Parasitism (Larval cyathostominosis, strongylosis)

Clostridiosis

Antimicrobial associated diarrhoea

NSAID toxicity (right dorsal colitis)

sand enteropathy

Carbohydrate overload

Salmonellosis

Parasitism (Larval cyathostominosis, strongylosis)

Sand enteropathy

NSAID toxicity (Right dorsal colitis)

Inflammatory of infiltrative disorders

IBD

Dietary: abnormal fermentation

Neoplasia

Lymphoma

Peritonitis, abdominal abscessation

signs of endotoxaemia

Depression

Tachycardia

Tachypnoea

Fever

Colic

Diarrhoea

Hyperaemic (toxic) mucous membranes

Usually see low white cell count, low neutrophil count, immature 'band' neutrophils

Treatment: polymyxin b, hyperimmune plasma, pentoxyfilline, flunixin

Moxidectin, fenbendazole

Can cause a plaque of oedema on ventral abdomen

Associated clinical signs

Colic due to pain

Muscle weakness and fasciculation's due to electrolyte loss (from intestinal mucosal ulceration causing plasma and blood loss)

Protein loss

weight loss and ventral oedema

Endotoxaemia

Vaculopathy

Laminitis

Ventral oedema

Pyrexia, depression, extended CRT, sweating

Tachycardia, altered mucous membrane colour, increased skin tenting (also caused by dehydration)

The increased borborygmi and diarrheic faeces is from decreased intestinal transit time and reduced water absorption from intestinal contents

Most cases in late winter and early spring

More common in younger animals (1-5 years old)

Approach to diagnosis

Clinical pathology

Red cell parameters considered in conjunction with protein concentrations will help to determine the extent of any dehydration

Anaemia

parasitic colitis

Gastric ulceration

White cell parameters

Extent of any endotoxaemia

Salmonellosis

Clostridiosis

Peritonitis

Total protein and albumin concentrations

Increased as a consequence of dehydration

decreased as a result of protein loss into the intestinal tract

Globulin concentrations may be increased as a result of dehydration or a chronic inflammatory response

Serum protein electrophoresis

helpful in distinguishing parasitic colitis, where an elevation in beta1 globulins may be appreciated

Severe diarrhoea will result in electrolyte losses

Sodium, potassium, calcium and bicarbonate especially

Direct visual inspection of faeces or microscopic examination of a wet preparation of faeces

often allow identification of cyathostome larvae in cases of parasitic colitis

Faecal worm egg count results should be interpreted with caution

the pathogenic stage of both large and small strongyles primarily involves immature parasites (not acquired ability to lay eggs)

both cyathostomosis and strongylosis may not be associated with a significant faecal worm egg count

faecal culture = vital step in identification of bacterial enteritis

e.g. salmonellosis, clostridiosis

Advisable to collect multiple samples over a period of at least 3 days

Does not mean cultured pathogenic bacteria is causative e.g. in cases of chronic diarrhoea where salmonella species are often cultured in association with a separate primary enteropathy

Rectal biopsy

samples should be collected from 10 and 2 o'clock positions to avoid the larger blood vessels in the dorsal midline of the rectum

Ultrasonography

Percutaneously through abdominal wall or per rectum

Sugar absorption tests

To assess small intestinal absorptive function

Most cases of diarrhoea involve changes to large intestine, but some involve small intestinal changes

Can be seen in some cases of parasitic colitis and intestinal lymphosarcoma and the infiltrative bowel diseases

Administer 1g/kg bodyweight of glucose via a stomach tube and then measure blood glucose concentrations over the following 4 hours

In a normal horse the blood glucose concentration reaches a peach of approx. double the baseline concentration at 2 hours

a reduced peak indicates a state of malabsorption and in an adult horse with diarrhoea, this suggests that there are significant changes present in both the small and large intestine

Surgical bowel +/- lymph node biopsy

histopathological examination of full thickness intestinal wall biopsies

can only be taken at laparotomy and usually requires general anaesthesia to allow samples to be collected from multiple sites along the intestinal tract

alternative: collect mesenteric lymph nodes biopsies (may reflect the pathological changes present in adjacent intestine)

can be performed laparoscopically in the standing animal

Initial pyrexia, depression and colic prior to onset of diarrhoea

profound leucopenia present at the acute stage

Salmonellosis often associated with previous stress or intercurrent disease

highly infectious and has zoonotic potential

isolate and barrier nurse

Rarely a cause of chronic diarrhoea

consider underlying disease process

often associated with start (or cessation) of antimicrobial therapy

other stresses e.g. transport, GA, withholding roughage may be risk factors

Both C perfringens and C difficile have been identified

Often younger animals (1-5) but can be seen in elderly

Weight loss may occur prior to diarrhoea

Usually occurs late winter and spring

Initial pyrexia and leucopenia are variable and faecal worm egg counts are often negative. Macroscopic faecal (or rectal glove) examination may identify larvae. Elevated Alpha and beta globulin concentrations may be seen.

Histopathological examination of a rectal mucosal biopsy can be diagnostic

Strongylosis usually a cause of colic or ill thrift. The condition may be associated with diarrhoea as a result of changes to blood supply in the large intestine. Faecal worm egg count may be negative.

Peritonitis

Diagnosis is confirmed by abdominal paracentesis and peritoneal fluid analysis. Aggressive treatment required.

Prognosis depends on underlying cause e.g. gut perforation, abscess, neoplasia

Lymphosarcoma

Typically insidious onset and slow progression and primarily affect small intestine.

Concurrent malabsorption and weight loss often occur.

Thickened bowel may be seen on rectal exam or USS

Dx - histopath of bowel wall or LN biopsy

Prognosis poor, but horse may respond to high-dose corticosteroids.

Hypoproteinaemia and hypoalbuminaemia develop rapidly and may present before the onset of diarrhoea

Usually causes an impaction colic

Diarrhoea often mild and intermittent

Acute deterioration associated with bowel wall inflammation and perforation

Sand is present in faeces and may be identified using sand sedimentation test.

Treatment

Maintain fluid and electrolyte balance once fluid deficit corrected

Fluid deficit (litres) = percentage dehydration x bodyweight (kg)

Maintenance fluid requirement (litres/day) = 0.05 litres/kg/bodyweight/day

The initial fluid deficit should be replaced within 4-12 hours and represents an extracellular fluid loss

Replacement with a balanced polytonic crystalloid fluid such as lactated Ringer's solution is most suitable.

In acute, severe cases, hypertonic saline may be used, but it is essential this is followed by an appropriate volume of polytonic crystalloid fluid to replace the extravascular extracellular fluid

If profuse diarrhoea continues more than 12 hrs

monitor calcium and potassium balance and supplement intravenous fluids if necessary

In severe cases of colitis, the loss of intravascular plasma proteins and reduced oncotic pressure results in fluid accumulation in the interstitial tissues (oedema) and it becomes difficult to maintain crystalloid fluids in the intravascular space

treatment = colloidal solution such as hetastarch or plasma

expensive tho

Caution with intravenous catheter care in diarrheic animals, as they are at an increased risk of complications e.g. jugular phlebitis and thrombosis

Continue until diarrhoea stops or fluid maintained with voluntary drinking.

Minimise endotoxaemia

reduce absorption of endotoxin

activated charcoal administered at 0.5 - 1g/kg by stomach tube once or twice daily.

Limit the effects of circulating endotoxin

Flunixin meglumine

0.25mg/kg intravenously 3x a day

Polymixin B

Endotoxin specific IgG

Prolong intestinal transit time

Codeine phosphate up to 3mg/kg orally 3x a day

Bismuth subsalicylate

up to 4 litres/500kg orally 2x a day

Antisecretory agents

Loperamide 0.1-0.2mg/kg orally 4x daily

Reduce bowel inflammation

NSAIDs

Flunixin (0.5-1.1mg/kg IV)

Corticosteroids

Dexamethasone 0.05mg/kg IV or IM

Nutritional support

Alfalfa or similar hay ad libitum

High protein and energy concentrate

Mineral supplementation

Turnout to grass can be beneficial in some instances

make all dietary changes gradually

General nursing

Isolation and barrier nursing if salmonella species or C difficile is suspected

Limb bandages/supports to prevent oedema accumulation

Tail protection

Perineal cleaning and protection with petroleum jelly to prevent scalding

Laminitis prophylaxis with frog supports and topical nitroglycerine

Specific therapy

Cyathostomosis/strongylosis

Anthelmintics

Fenbendazole (7.5-10mg/kg orally once daily for 5 consecutive days)

Ivermectin

0.2mg/kg orally

moxidectin

0/4mg/kg orally

Clostridiosis

Metronidazole 15mg/kg orally 3x a day

probiotics/yoghurt may be beneficial

Grain overload

nasogastric intubation to allow gastric decompression

Epsom salts (magnesium sulphate) 1g/kg by stomach tube

Aspirin 10mg/kg orally or IV once daily

Sodium bicarbonate intravenously if indicated by blood gas analysis

Laminitis prophylaxis (frog supports and topical nitroglycerine)

Peritonitis

Broad spectrum bactericidal antimicrobials 3 x daily in combination with gentamicin 2.2mg/kg IV 3x daily or 6.6mg/kg IV once daily

Sand enteropathy

Psyllium mucilloid 0.25-0.5kg for a 500kg horse in 4-8 litres of water by stomach tube

Ispaghula husk 300-400g for a 500kg horse in 4 litres of water by stomach tube

NSAID toxicity

Psyllium mucoid 30-60g administered in feed for 3-6 months

Misoprostal orally

decrease gastric acid secretion and increase recovery of the ischaemia-injured equine jejunum

Sucralfate 22mg/kg orally 2 or 3x daily

Metronidazole 10-15mg/kg orally 2x daily

Potomac horse fever

Tetracyclines 6/6mg/kg slowly IV once daily for 3-5 days

Prognosis

Better

Worse

Intermittent diarrhoea

Soft or cow pat faecal consistency

No or mild endotoxaemia

Animal able to maintain fluid balance

Voluntary drinking and eating continues

good response to correction of fluid/electrolyte imbalances

Diarrhoea resolves within 48 hours

Albumin concentration maintained

no secondary complications develop

Persistent diarrhoea

liquid faecal consistency

severe endotoxaemia

animal unable to maintain fluid balance

voluntary drinking and eating ceases

fluid/electrolyte balances continue

diarrhoea continues beyond 48 hours

hypoalbuminaemia develops

secondary complications such as thrombophlebitis, laminitis and septicaemia develop

Ventral oedema (increased hydrostatic pressure and vascular permeability, decreased oncotic pressure and lymphatic drainage)

Oxytetracycline only early in disease process, once colon inflamed it increases the permeability of the vasculature

Intracellular bacteria therefore penicillin/gentamicin will not work

Identification by bacterial culture or PCR analysis

103 colony-forming units/g feces or intestinal contents; demonstration of enterotoxin or cytotoxin A or B (for C difficile)

Slower onset, oral cavity ulcers; early ventral edema associated with hypoproteinemia; gastric or intestinal ulceration

Abdominal discomfort from intraluminal sequestration of fluid or gas, or 2ry to inflammatory mediator activity

Can be mild e.g. recumbency or inappetence or severe such as rolling and thrashing

Neorickettsia risticii is the causative agent which causes biphasic fever, laminitis, and colitis of variable severity

N risticii-associated colitis may be supported by comparing paired serum titers using immunofluorescence assay testing techniques

Diagnosis confirmed using antigen detection by identification of N risticii morulae in WBCs during the acute phase of the disease, isolation of the organism from WBCs or PCR testing of WBCs or faeces

Equine coronavirus can be a cause of acute equine colitis, and faecal PCR for equine coronavirus should be included in the screening process

Horses with acute colitis absorb large quantities of endotoxin across the disrupted intestinal mucosal barrier

High risk for developing laminitis, thrombophlebitis and disseminated intravascular coagulation

digital pulses should be monitored 3-4x daily until systemic signs of colitis abate

Endosperm is a hyperimmune serum obtained from horses vaccinated against salmonella typhimurium Re mutant (diluted in sterile isotonic saline or lactated ringers solution)

dilution at 1:10 or 1:20 to minimise the risk for immune mediated hypersensitivity reactions

Risk potential for nephrotoxic effects so use with care and not recommended in azotaemic horses

recommended to prevent endotoxin-induced prostanoid synthesis in horses

Compared to other NSAID regimens it has a lower risk of adverse effects including gastrointestinal ulceration, ileus and renal papillary necrosis

Firocoxib (cyclooxyrgenase-2 inhibitor drug)

fewer adverse effects

Antiinflammatories and analgesics

Flunixin meglumine (1.1mg/kg, IV, every 12 hours)

Phenylbutazone (2.2mg/kg, IV, every 12 hours)

can contribute to further damage of the intestinal epithelial barrier, so lower and less frequent does should be considered

Firoxocib

selective cyclooxyrgenase 2 inhibitor so safer in horses with similar beneficial anti-inflammatory effects

Must balance need for analgesic effects of non selective NSAIDs against the possibility of further bowel damage induced by blocking the protective effects of intestinal mucosal prostaglandins

Endogenous prostaglandins are important inhibitors of intestinal inflammation, an blocking these agents with nonselective NSAIDs may slow recovery and healing of inflamed intestinal mucosa

Analgesic combination with minimal effects of gastrointestinal motility

butorphanol (0.96-0.1mg/kg IM) and detomidine (0.01-0.02mg/kg IM)

Antimicrobials

Broad spectrum antimicrobials may be considered for profound/persistent neutropenia and risk for complications associated with sepsis, such as peritonitis, pneumonia, cellulitis, thrombophlebitis and disseminated intravascular coagulation.

Potassium penicillin G + gentamicin common regimen for horses with ongoing systemic disease

Oral broad spectrum antimicrobials are not recommended due to potential for further disruption of intestinal microbial population

Oral administered metronidazole (10-15mg/kg every 8hrs) may be indicated in horses in which Clostridium app are suspected to play a pathogenic role in disease

May have local anti-inflammatory effects and can cause loss of appetite in some horses

Most horses with acute colitis are partially or completely inappetent and lose protein due to cachexia and protein losing enteropathy

Partial or total parenteral nutrition may be indicated in horses that remain inappetent for more than 3-4 days

Frequent complications include laminitis, thrombophlebitis, debilitation and marked weight loss