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Complex I study (Enzyme assay (NADH: DCQ, NADH:HAR, NADH:DCQ (redox), NADH…
Complex I study
Enzyme assay
NADH: DCQ
NADH:HAR
NADH:DCQ (redox)
NADH:HAR (redox)
Citrate synthase
Coupled complex II and III
Ratiometric redox analysis
ND3
NDUFS1
NDUFV1
ND5
NDUFA9
NDUFS2
Hr-CNPAGE
Assembly
Selective reduction
Complex I total (ox)
Complex I total (red)
Complex I total after selective reduction
Immunocapture/electroelution
Complex I subunits (red)
Complex I subunits (ox)
SDS PAGE/ Western blot
Verification of pAB and antibodies
Graphics
Ratiometric redox analysis