:microscope: PCR (Polymerase Chain Reaction) :female-scientist::skin-tone…
PCR (Polymerase Chain Reaction)
:star: Forensics use very often this technique to find criminals with just mucus, saliva samples, nails or hair follicle.
:star: PCR is helpful to find bacteria and viruses (as COVID-19, nowadays).
:star: Through PCR, we can create lots of copies of DNA fragments.
:star: The DNA Thermal Cycler is a machine very expensive and useful. This because it can change it´s temperature vary, many times in some hours.
:star: The DNA polymerase used here, is special because it supports high hieat (that is produced during the reaction).
:star: At 50°C, both primers take their places in each strand.
:star:At 95°C, double-stranded DNA separates. Breaking the hydrogen bonds. Leaving single-stranded DNA.
:star: After cycle 3, we get some of the desired fragments (copies of just the targeted sequences on the DNA).
:star: At 75°C, DNA polymerase start to attach nucleotides to each strand.
:star: The total cycles that the DNA Thermal Cycler take to finish PCR is 30.
:check: Extracted DNA sample ( from blood, saliva, mucus, hair follicle, etc)
:check: Two primers
:check: PCR tube
:check: Micropipette tips.
:check: DNA polymerase
:check: DNA Thermal Cycler
Use the micropipette to suck some extracted DNA and deliver it into the PCR tube.
Still with that tool, add some primer 1 to the PCR tube.
Do the same as the previous step but with the primer 2 and the DNA polymerase.
Place the PCR tube into the DNA Thermal Cycler. Set the device so it starts the process.
Wait some hours for the DNA Thermal Cycler to finish.
Analyze the results
PCR. Learn.genetics.utah.edu. (2020). Retrieved 30 March 2020, from
:warning: Don´t forget to change the tip when you already used it.