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Dental plque (Host Factor (GCF (gingival crevical fluid) fluid flow…

- - - - Inhibit growth and facilitate the clearance of some exogenous micro-organisms
      - Can act as primary nutrition source to oral microbes
      - Absorb to surface forming acquired pellicle
    - - Lysozyme: lyse bacteria
      - Lactoferrin
  - - - Leukocytes that can phagocytose bacteria
- - - - Inorganic materials
      - eDNA
      - Polysaccharides
      - Salivary and microbial proteins
    - - Bulk fluid layer above the biofilm
      - Presence of channel within the biofilm that aids in diffusion of nutrients and removal of wastes
      - Lower layers consist of a dense layer of micriobes attached to the surface and bound together by extracellular matrix
      - Biofilms are capable of responding to changes in environmental conditions
    - - Mutualistic
        
        P.gingivalis and T. denticola in planktonic
        (both need each other to survive)
      - Antagonistic
      - Synergistics
        
        P. gingivalis and T. denticola in biofilm
        （both organism can survive on their own but grow better when together）
      - Metabolic interaction
        
        Metabolic excreted by one could be used by the other
        
        One organism break down substrate, creating available substrates for different organisms
      - Commensalism
        
        P.gingivalis can (only) live in environment without CO2 when co-culture with F. nucleatum
        (One species gain benefits while the other neither benefits nor harmed)