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biology CH6-7 (organells/animal (endoplasmic reticulum (rough (Is…
biology CH6-7
organells/animal
lysosome
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It is made of hydrolytic enzymes that break down macromolecules like nucleic acids, proteins, and polysaccharides
endoplasmic reticulum
Called the "intracellular highway" because it is for transporting all sorts of items around the cell.
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rough
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Made of cistern, tubules, and vesicles. And its called “rough” because it has ribosomes attached to its surface.
series of connected flattened sacs, found ind eukaryotic cells and it’s main function is to produce proteins.
smooth
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it is called smooth because it is not studded with ribosomes and is associated with smooth slippery fats
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golgi apparatus
Make, process and package proteins
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function
An organelle in eukaryotic cells that stores and modifies proteins for specific functions and prepares them for transport to other parts of the cell.
Chemical products transported have sealed droplets or sacs called vesicles and move to a ‘deliveries only’ part of the Golgi.
And then the vesicles are delivered into the ‘unloading bay’ of the cis Golgi network (here the goods received are checked over) they come from the ER to the Golgi or another part of it.
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vacuole
Used for storage, vacuoles usually contain water or food.
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ribosomes
make protein
the bigger submit is whereto amino acids are included can be found floating within cytoplasms or attached to the endoplasmic reticulum
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They function to synthesize proteins and are made of proteins and ribonucleic acidic almost equal amounts.
plant cells
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chloroplast
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They absorb sunlight and use it conjunction with water and carbon dioxide gas to produce food for the plant.
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has a inner and outer membrane with an empty intermediate space in between, inside, it has stacks of thylakoids,
grant as well as stroma, the dense fluid inside.
mitochondria
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the fluid contains Is Called matrix. It is the powerhouse of the cell it preforms cellular respiration
Its made of two membranes the outer membrane covers the organelle and contains it like a skin. The inner membrane fold over many times creating layered structures called cristae,
takes in nutrients from the cell and breaks it down, turning it into energy.
nucleus
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The “brains” of the cell, the nucleus directs cell activities
nucleolus - made of proteins and.ribonucleic acids and it transcribes ribosomal RNA and combines it with proteins to form incomplete ribosomes
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The nuclear membrane are phospholipid bilayers which are only preferable to small non polar molecules and because this allows transport
nuclear pore - regulate movement of molecules from the nucleus to the cytoplasm snd vice velds like they permit passage of water ,ions, ATP
cells
eukaryotic
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found in animals, humans, plants, fungi, insects
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cell fractionation
then its poured into another tube at another speed ( 20,000) g (mitochondria or chloroplast if it was a plant).
hen another tube at another speed (80,000) microsomes/ pieces of plasma membranes and cells internal membranes. then at (150,000 g) ribosomes
But for each time spinner harder it will be a longer time 10,20,60 minutes and last one was 3 hours to get those results
The resulting mixture (homogenate) is centrifuged (spinned) at starting (1,000 g) (nuceli and cellular debri)**G is times the force of gravity
used to separate cell components based on size and density , by cells being homogenized in a a blender to break them up.
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types of microscopes
Phase contrast
fluorescence
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the locations of specific molecules in the cell can be revealed by labeling the molecules with fluorescence dyes or antibodies
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Confocal -
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using a technique called “optical sectioning’ eliminates out of focus light from a thick sample creating a single plan in the image
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