Phlebotomy Ch.13: Lecture 2

Artery Puncture

  1. Label and ice specimen

make sure to dispose of needle in the sharps container

label specimen with waterproof pen and place in ice slurry and deliver to the lab immed.

Complications

Arteriospasm

constriction of artery due to pain, preventing O2 from reaching tissue

nerve damage

caused by accidental contact with a nerve

Hematoma

caused by going through the artery or holding inadequate pressure

Thrombosis

clot formation within artery

Hemorrhage

can occur in pts receiving anticoagulantion therapy

infection

can occur from skin contamination and be carried to rest of the body

Sampling Errors

they must be chilled if not analyzed within 30 minutes to prevent gas loss

using too much heparin lowers pH

using too little heparin can cause clotting

inadequate mixing can cause clotting

air bubbles decrease CO2 values

improper syringe causes gas loss

pH alters with use of incorrect anticoagulant

puncturing a vein instead of an artery

contamination with room air after collection

Specimen Rejection

specimen quantity is not sufficient

specimen is clotted

improper or no label

use a wrong syringe

air bubbles in the specimen

failure to chill if not testing within 30 minutes

specimen is delayed too long to be tested

Capillary blood gases

alternative to ABG's especially in pediatric pts

not as desirable b/c capillary blood is a mixture of venous blood, arterial blood and tissue fluid

collection technique also allows for exposure to room air before sealing

CBG procedure

use normal heel stick procedure except warm for 5-10 minutes to make blood more arterialized

use heparinized capillary tube; some may require a metal flea to be inserted prior to collection

fill completely with blood and seal ends

mix contents using the magnet and flea

transport chilled until analyzed