ChIP-MS
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Delphine Priority 1-2
iST
HDAC2 Priority 2
NRF Priority 2
CTCF Priority 1
cell type (K562 vs HeLa)
Additional check (QC) of samples prior MS
systematic check and comparison of protein/DNA amount prior IP, after IP and after iST
masking the specific signal by Ab ?
WB
PAGE or/and BA, prior iST?
qPCR/DNA yeild
Himanshu vs Pinar workflow
PAPER Priority 3
SICAP Priority 2-3
Ab issue?
locus issue?
pA vs pG
cell type
Chromatin prep (frozen vs fresh, Fixation, amount of proteins/DNA per IP etc)?
speed vac
de-cross-linking
washes
Ab batch?
understand each step of the protocol
apply on the validated model