ChIP-MS

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Delphine Priority 1-2

iST

HDAC2 Priority 2

NRF Priority 2

CTCF Priority 1

cell type (K562 vs HeLa)

Additional check (QC) of samples prior MS

systematic check and comparison of protein/DNA amount prior IP, after IP and after iST

masking the specific signal by Ab ?

WB

PAGE or/and BA, prior iST?

qPCR/DNA yeild

Himanshu vs Pinar workflow

PAPER Priority 3

SICAP Priority 2-3

Ab issue?

locus issue?

pA vs pG

cell type

Chromatin prep (frozen vs fresh, Fixation, amount of proteins/DNA per IP etc)?

speed vac

de-cross-linking

washes

Ab batch?

understand each step of the protocol

apply on the validated model