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markerless deletion of an enterotoxin gene cluster in B.cereus (methode,…

- - - - reduce chemical fertiliser on the environment
      - using in maize, rice, wheat, bean, soybean
        
        The plant genotype shapes PGPR communities both in terms of pop- ulation size and composition (Bouffaud et al., 2011; Haichar et al., 2008; Hartmann et al., 2009).
      - stimulate plant growth
      - suppressing plant pathogens
      - accelerate nutrient
      - biocontrol
      - improve nutrient uptake
      - degrade soil pollutant
      - alleviation of the salinity stress effects
      - seed germaination
      - yield
    - - direct
        
        N
        
        P
        
        phytohormone
        
        IAA
        
        gibberellin
        
        ethylene
        
        cytokinin
        
        abscisic
      - indirect
        
        siderophores binding
        
        Fe3+
        
        Pb
        
        Cr
        
        Al
        
        actinide ions
        
        antifungal
        
        siderophore
        
        lytic acid
        
        quitinase
        
        protease
      - interact : use phytohormone for interact with plant
        
        flavonoid from plant
        
        nod factor from bact : chemotaxis
        
        exudate is role of interaction
        
        co-adaptation of the two partners, leading to specific interaction between plant and PGPR,
        
        secretion of flavonoids by leguminous plants is increased after inoculation of A. brasilense and Chryseobacterium balustinum (Volpin et al., 1996; Dardanelli et al., 2010).
        
        role
        
        implicated in plant genetic : change of 2' metamolism
        
        plant and bact surface component
        
        specific colonization of plant root
        
        colonizes root hair then extend to the whole root
        
        week adherence : protein
        
        strong adherence : polysaccharide
        
        potential of root adsorption
        
        protein function like adhesion
        
        plant not passive target
        
        pathogen interaction
        
        virus factor
    - - colonise the rhizosphere of plant
      - take exudate from plant
      - research report
        
        Azospirillum, Bacillus, and Pseudomonas, and the fungus Glomus
  - - - cooked vegetables and meats, boiled or fried rice, vanilla sauce, custards, soups, ice cream,cooked vegetables and meats, boiled or fried rice, vanilla sauce, custards, soups, ice cream, Keith R. Schneider, Mickey E. Parish, Renée M. Goodrich and Taylor Cookingham2
        FSHN04-05
        (2004)
    - - cytk
      - nhe
      - hbl
    - - heat -labile toxin
        
        diarrhea
        
        4-16 h incubation time
        
        12-24 h express symtom
      - heat-stable
        
        vomit
        
        occur 1-5 h
    - - (80 min at 121°C and 60 min at 150°C at pH 9.5) inactivation of cereulide A. Rajkovic1, M. Uyttendaele1, A. Vermeulen1, M. Andjelkovic2,3, I. Fitz-James4, P. in ‘t Veld4,
        Q. Denon1, R. Ve ́rhe3 and J. Debevere1(2007)
- - - - 3' exonuclease
      - polymerase
      - ligase
    - - overlapping
      - sequence
        
        overlap
        
        assembly of adjacent fragments
        
        gene-specific for template priming
      - step
        
        create complete final sequence
        
        mark overlap position
        
        choose 15-25 overlap region
    - - set up reaction master mix
      - incubation
      - 3.transformation
  - - - positive selection system