Allowed 15 min to explore the tank, before a model predator was placed in the tank, 14 cm long, which looked similar to the pike cichlid ( C. alta)

Observed how the test individual interacted with the predator. Whether it was watching the predator the whole time, or trying to avoid it.

Inspection Behavior also used one plastic bottle with a school of 6 guppies in it.

Schooling Behavior was measured using 2 plastic bottles. One with a school of about 6 guppies, and the other empty.

This was tested for each sex and population with a max of 24 times

The River Aripo and River Turure both schooled less, being upstream with low predationm verusus the downstream high predation relatives

Oropuche had lower schooling tenancies, with the Quare females, when looking at other populations with high predation

The behavior was also tested to see if the guppy was on the same side as the school of fish

The Aripo and Turure who are normally found downstream, stayed on the same side as the school of guppies. As opposed to the upstream relatives who spent most of the time on the other side of the tank

The Quare and Oropuche guppies remained mostly on the school side, versus the other guppies from high predation populations

In all populations, all females where more apprehensive than the males to approach the predator.