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Planing Thesis (To-do list (Q2: ไม่รีบแต่สำคัญ :red_flag: (Conceptual…

- - - - Normal control
      - CCA control
      - 5-FU (Positive control)
      - High dose AL: Beta-eudesmol compound
        
        ทำไมถึงเลือก
        
        inhibiion tumor growth
        
        prolong survival time
        
        mind side effect
      - ทำไมต้อง Hamster
        
        BAX, Akt/PKB, p53, and caspase 9 expression level
        
        อ้างอิงจาก paper อาจารย์ตุลย์
- - - - p53
        
        Cancer: inhibits p53 :check:
        
        Anti-cancer: enhance p53 expression :check:
        
        General properties
        
        Stimulate of pro-apoptotic :check:
    - - Effector caspase protein
        
        Caspase 9 :check:
        
        Cancer: inhibits caspase 9 :check:
        
        Anti-cancer : enhances caspase 9 protein :check:
        
        Gerenal properties
        
        indicates mitochondria or intrinsic apoptotic pathway :check:
        
        Caspase 3/7 is final step both in intrinsic and extrinsic pathway
        :silhouettes:
        
        Caspase 8: indicates extrinsic pathway :silhouettes:
      - Anti-apoptotic
        
        Akt/PkB
        
        Cancer: enhance Akt/PkB expression :question:
        
        Anti-cancer: inhibits Akt/PkB expression :question:
        
        Bcl-2 regulator cell apoptosis and binding Bax protein :silhouettes:
      - Pro-apoptotic protein
        
        Bax :check:
        
        Cancer: inhibits BAX exprssion :check:
        
        Anti-cancer: enhance Bax expression :check:
  - - - VEGF :silhouettes:
        
        Anticancer: inhibits VEGF expression :check:
        
        Cancer: VEGF expression leading to angiogenesis :check:
      - ERK :silhouettes:
        
        Anti-cancer: inhibits ERK expression :check:
        
        Cancer: ERK expression leading to angiogenesis :check:
    - - E-cadherin
        
        Cancer: loss or reduce expression of E-cadherin :check:
        
        Anti-cancer : enhance expression of E-cadherin :check:
        
        General properties
        
        reduce interaction of cell-cell adhesion molecule :star:
        
        Malignant cells to escape from the primary tumor site
        
        Host cell indicators :!!:
      - TGF-beta
        
        General properties
        
        TGF-beta (reporter gene) bindding
        
        Smad pathway :check:
        
        Smad 4 protein :silhouettes:
        
        Non-smad pathway
        
        MAPK pathway
        
        C-Ski interfere :no_entry: :silhouettes: leading to SMAD4 formation :check:
      - EMT marker :star: :question:
        
        Eplithelial marker
        
        E-cadherin :check:
        
        Mesenchymal marker
        
        N-cadherin
        
        Fibronectin
        
        Vimentin
        
        Collagen
        
        EMT transcription
        
        Snail
        
        Slug
        
        ZEB1
        
        ZEB2
        
        Twist
- - - - in vitro
        
        Intrinsic pathway
        
        p21, p53 :check:
        
        Caspase 3,8,9,7 :check:
        
        Bax and Bcl-2 :check:
        
        inhibits CCA through Bax expression :check:
      - in vivo :question:
    - - Invasiveness and migration :question:
        
        in vitro :warning:
        
        in vivo :warning:
      - Angiogenesis
        
        in vitro
        
        VEGF and bFGF activation of ERK1/2 were blockage :check:
        
        in vivo :warning:
  - - - Apoptosis
        
        in vitro
        
        in vivo
      - Metastasis
        
        Angiogenesis
        
        inhibits VEGF-VEGF 2 signaling pathwy
        
        inhibits Snad-independent BMP
        
        inhibits p38MAPK, ERK1/2 and JNK 1/2/3
        
        Invasiveness and migration
        
        inhibits MMP-9 and MMP-13 expression
    - - Apoptosis
        
        induces apoptosis pathway through mitochondria pathway :star:
        
        Decrease anti-apoptotic
        
        Bcl-2
        
        increase pro-apoptotic
        
        Bax
        
        Increase effector caspase
        
        caspase 3
      - Metastasis
        
        migration and invasion
        
        Epithelial marker
        
        increase E-cadherin
        
        Mesenchymal marker
        
        Alpha-SMA
        
        N-cadhein
        
        Vimentin
        
        inhibits MMPs expression
        
        MMP-2
        
        MMP-9
        
        TGF-beta1
        
        Reduce Smad-independent TGF-Beta pathway
        
        Smad 2/3
        
        Reduce other pathway
        
        p38/MAPK
        
        ERK1/2
        
        JNK1/2
- - - - AMRESCO Code: E174-25G
    - - Buffer คงสัดส่วนไว้ แต่เพิ่มปริมาณ Enzyme แทน
    - - ทำให้ concentration เท่ากัน :!!:
  - - - Realtime PCR
        
        BIORAD :
      - Thermocycler
        
        Biometra Serial 1701118
      - Nanodrop :question:
      - Centrifuge :check:
        
        Hettich 320R
      - Microcentrifuge :check:
        
        Eppendrof serial 30491
    - - 96 well-Realtime PCR plate
        
        BIORAD :
      - Eppendrof 1.5, 1.0 and 0.6 mL :check:
        
        Axygen
        
        Eppendrof 1.5 mL Lot: 23516403
        
        Eppendorf 1.0 mL Lot: 32317119
        
        Eppendrof 0.6 mL Lot: 28813383