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Energy Metabolism: Photosynthesis (Light-Dependent Reaction (nature of…
Energy Metabolism: Photosynthesis
Light-Dependent Reaction
nature of light
short wavelength
high energy
gamma rays, cosmic rays, uv light
visible light (350 to 760 nm)
PAR
plants use it for photosynthesis
nature of pigment
pigments
absorb certain wavelength
get their color
chlorophyll a
absorbs red and blue
does not use high energy quanta
cause the electron to be excited
can be used in chemical reaction
if not used, return to original state
absorption vs. action spectrum
Fluorescene
light released by pigment
Accessory pigment
absorbs wavelength not absorbed by chlorophyll a
pass it to chlorophyll a
common types on land plants
chlorophyll b
good at resonance
carotenoids
poor at resonance
transfer only about 10% of absorbed energy
role is to protect chlorophyll a from excessive light
photosynthetic unit
photosystem I (P700)
less number of chlorophyll b
absorb red light of 700 nm efficiently
Fx
membrane found electron acceptor
contain iron and sulfur group
accepts electron from P700
reduce it than pass it to ferredoxin
ferredoxin
located at thylakoid membrane
reducing agent
pass the electron to enzyme
ferredxin-NADP+ reductase
reduces NADP+ to NADPH
photosystem II
chlorophyll a and b are equally present
reduces P700
plastocyanin
donates electron to the chlorophyll of P700
receives new electron from cytochrome b6/f complex
Q complex
donate electron to cytochrome b6/f complex
receives electron from phaeophytin
P680
receives electron from water molecule
donate it to Q complex
ATP Synthesis
chemiosmotic phosphorylation
Thylakoid lumen
house enzymes and electron carries
membrane is not permeable to protons
ATP synthetase
ADP+Pi -> ATP
Stroma Reaction
Calvin-Benson Cycle
occurs in stroma
RuBP
molecule acceptor
contains carbon
3 phospoglycerate
product of RuBP+CO2
2 identical molecules
RUBISCO
enzyme that carries the reaction RuBP+CO2
one of the largest and complex protein
can constitute up to 30% of protein in a leaf
1,3-diphosphoglycerate
reduced by NADPH
3-phosphoglycerate
receives high electron from ATM
Anabolic Metabolism
build up of large, complex molecules
synthetic pathway of polysaccharides and fats
Gluconeogenesis
anabolic synthesis of glucose
PGAL converts to dihydroxyacetone
condense with 3-phosphoglyceraldehyde
form fructose-1, 6-bisphosphate
lose of phosphate
fructose-6-phosphate
glucose-6-phosphate
Enviromental and Internal Factors
Light
#
quantity
intensity and brightness
clear day= more photosynthetic activity
plants near equator receive more sunlight
summer time
light is too intense
protective measurement is necessary
duration
numbers of hours that sunlight is available
locations play huge role
equator
12 hrs of sunlight throughout the year
near the poles
24 hrs of daily sunlight during midsummer
mid lattitude
short winter days
little photosynthetic activity
long summer days
more photosynthetic activity
quality
wavelength
color
blue light
deflected upward at dawn
red light
small impact on photosynthesis at dawn
leaf structure
#
temperate and tropical plants
palisade parachyma above/spongy mesophyll below
absorbs CO
poor at water conserving
plants in hot,dry habitat
tightly packed leaf cells
water conservation
inefficient in dissolving CO2
cylindrical leaves
minimum water loss
water
open stomata during day time
carbon dioxide entry
water loss
close stomata at night
no carbon dioxide activity
retain water
C4 metabolism
plant's water use efficiency
water loss per molecule of CO2 absorbed
low ratio
photorespiration
wasting process
carbon absorption and concentrated in leaf
Energy and Reducing Power
energy carriers
photosynthesis
converts light energy to chemical energy
use lights captured by pigments
guanosine triphosphate
helps ATP to enter and drive the chemical reactions
photophosphorylation
involves light energy
substrate-level phosphorylation
ADP--> ATP
oxidative phophorylation
ADP phosphorylated to ATP
reducing power
oxidized state
lose of electron
do not carry as many electron as it supposed to
usually contain oxygen
reduced state
gain of electron
usually contain hydrogen
reducing power
forced electrons onto compounds
electron carriers
#
cytochromes
contains cofactor heme, iron group
carries electron to short distance
plastoquiones
transport electron within membrane
dissolve easily into the lipid component
plastocyanin
contains copper group
associated with chloroplast membranes
Terminology connection
significant of light
leaf properties