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PRODUCTION ON INSULIN (TECHNIQUE (The genes are (Cut by the Restriction…
PRODUCTION ON INSULIN
TECHNIQUE
The genes are
Cut by the Restriction Enzymes
Linked to the plasmids by the Dna Ligase
Insulin genes linked to the B- galactosidase gene
Carries a promoter for the transcription
Recombinant plasmids
Inserted back in the bacteria
The gene is chemically syntesized
Insulin A chain
Inserted in an E. coli plasmid
Insulin B chain
Inserted in an E. coli plasmid
THE ISSUE
Treatment
Injections of insulin
In the past
cons
Severe allergic reactions
Allergic reactions to its contaminants
Expensive
insulin taken from cows and pigs pancreases
Type I diabetes mellitus
metabolic disease
lack of insulin
INSULIN PRODUCTION IN SACCHAROMYCES
The insulin gene is inserted in the Yeast cell
The proinsulin is
folded
cleaved
Functional protein is produced
produced
Cheaper and quicker than using E.coli cells
FURTHER APPLICATIONS
Interferon
Factor VIII
Growth hormon
OUTCOMES
Insulin A and B chains joined with B-galactosidase
Purified
Mixed
Extracyted
Connected
Disulfide cross linkages
Form the functional protein