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Microscopes (TEM (E- gun :arrow_right: beam of e- :arrow_right: focused…
Microscopes
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OM
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+Live specimen, stained in colour
-Thin specimens, lowest mag + res
SEM
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+Higher mag + res than OM. Produce 3D image, don't need thin specimens--- e- don't have to pass through.
-Lower res + mag than TEM, dead specimen--- vacuum. Not in colour.
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Light microscopy limits to res + mag
E- microscopes= greater resolving power as e- have shorter wavelength than light