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Vaziri - Dynamic maps of neural circuits (2f microscope (what resolution…
Vaziri - Dynamic maps of neural circuits
Imaging in scattering tissue
c elegans
zebra fish
mice??
2f microscope
500 um cube
5 Mpix
2.5Ghz
dwell time of 0.4 ns... lifetime
what resolution can we get away with
5um cell?
larger spot?
kill axial resolution
light sculpting
decouple axial and xy resolution
temporal focusing
chirping?? not really actually
1 Mpix
5Mhz
200 ns dwell time
what is optimum?
resolution
5 um
what thye use
6000 neurons at 17Hz
12000 at 5 Hz
confirmed with auditory correx frequency mapping
thermal
15 mW
use delay lines to increase rep rate
slightly reduced sensitivity but same presion
3 photon?
Just for deep regions
hybrid technique
recorded at 78 hz
1.2 mm deep
400 neurons at 4 Hz
A lot of brain still to image
how valuable would it be to do the whole brain
maybe 10% would be almost as valuable
different regions simultanously
how many neurons give you 95% of the information