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Tommas Patriarchi (Other Tools (monitor in real time (micro dialysis…
Tommas Patriarchi
Other Tools
Peptidtes
Neurotransmitters
neuromodulators
monitor in real time
cyclic voltometry
micro dialysis
slow!!
use fiber photometry to record
can monitor dopamine concintration in vivo
can pick out individual cellular events
bridges the gap between other thechniques
Can also do imaging
locomotion
reward consumption
can discriminate accross layers
lots of other neurotransmitters
multi colour imaging
Ca and dopamine together
Glutamate and Dopamine together
glutamate goes down
Dopamine and NE sensor
red and yellow for dopamine
seems photostable
GCaMP
Almost perfect
Need something new?
G-Protein Coupled Receptors
how bio does it
introduce GFP
dopamine sensor
worked for lots of sensors
delight
similar to cyclic voltometry
Flim... mentioned at the end
FRET based sensor?
Photometry devices!!!!