Valentian Emiliani - Circuit optogenetics (thermal damage (how to measure,…
Valentian Emiliani - Circuit optogenetics
soma targeted opsin
can generate up to 50 spots in 3D
with resolution of the size of a soma
volume of 1 mm3
liquid crystal refresh rate
read activity then replay with optogenetics
Galvo with static holigrams
no longer limited by liquid crystal refresh rate
now limited by the illumination time of the opsin
im not sure about this...
what does it do to your resolution?
apparently this is OK
does it limit what you can do?
why not just do it all with galvos??
parking galvo issue?
furier heat transfer - look up
single spot - 0.3 deg
100 spots still less than 1 deg
thermal diffusion lenght 50 um in 3 ms
keep the illumination short ms
how to measure
Er-Yb co doped glass particles
good agreement in agarose
glue at the end of a fiber
very presise inviv temperature measurement