ofer yizhar
high throughput optical mapping
of prefrontal cortex connectivity
past experierence
behavioral states
contex
Decesions
very connected region
fear
amygciala
extintion of fear
right beside each other
found a way to change the contribution of amygdala cells in the pfc
changed how a memory was formed
still had context fear but not out of context fear
study where a tone was paired to a foot shock
want to look at the local circut to fully understand
techniques to do this are very low throughput
can he develop a high throughput method
optogentics
retrograde staning
click to edit
2 photon imaging
z stack
specific targeing
localise it on the soma
spirialing on the soma
greater photocurrent
GCamP
same wavelenght
can detect cell activity
works
confirms some information about cortical connectivity
however, in amgdella most connections are come from layer one
connection probability low
Can look at strength of connection
strongest connections are coming from cells more supperficially