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Stephane Dieudonne - Voltage Sensing (if you record from a volume its slow…
Stephane Dieudonne - Voltage Sensing
All electrodes and no brain
if you record from a volume its slow
Jumping spot to spot... Random access
Accustic optic deflections
RAMP
How to adapt to voltage recording
Problem
Low Photon Flux
Need very efficient f-molicules
high photo bleaching
low f molicule expression
Solution
Scan around the membrane surface
similar to spiral paterns for ChR2
playing with the ao modulator you can create a dounut, spiral etc
Playing around with the holographic patern
Dirac pattern
can sequentially access points
movement artifact is reduced
photobleaching is massively reduced, 1% per minute
Does it work with voltage sensors
ASAP3 50% df/f
not fast enough for full spikes
3-5 minutes at 3-5 kHz
Can work down to layer 5
Can separate different neuron populations
Further pulse shapping
Reduces movement artifacts
Neurophil contamination
Can be subtracted